Jotbody developed a time-saving and cost-effective professional platforms for the breeding and immunization of camel, llama, alpaca to guarantee sdAb of excellent quality.
We care about Animals
我們關心動物
Our first commitment is to ensure that our animals are properly nourished. Our staff is dedicated to providing a comfortable and well-equipped environment that ensures the highest welfare and ethical standards for animal care.
我們首先承諾是確保我們的動物得到良好的飼養。我們的員工致力於提供一個舒適和設備完善的環境，以確保最高的福利和道德標準的動物護理。
Scientists and staff at Jotbody are highly experienced on immunization of a variety of animals. Their knowledge is indispensable on generating high-quality sdAb since the high titer in the animal blood is the first step to guarantee the success of sdAb isolation.
Jotbody的科學家和工作人員在多種動物免疫方面經驗豐富。他們的知識和經驗對於產生高品質的納米抗體是必不可少的，因為動物血液中的高滴度是保證納米抗體分離成功的第一步。
Our team is ready to discuss with you every step of our workflow from animal immunization to PBMC isolation
Our general workflow includes up to 4 and 7 times of immunization with the antigen of choice for camelids and shark respectively. After the last immunization, our staff collect blood samples from the immunized animals and isolate the peripheral blood mononuclear cells for further analysis. The whole immunization workflow takes 1-2 and 3- 6 months for camelids and shark respectively.
我們的一般工作流程包括分別用於駱駝和鯊魚的多達4次和7次的抗原免疫。最後一次免疫後，我們的工作人員採集免疫動物的血液樣本，並分離外周血單核細胞進行進一步分析。整個免疫流程對駱駝和鯊魚分別需要1-2個月和3-6個月。
Workflow for camelids immunization
羊駝免疫流程
Day ~2, analysing antigen quality and analysis report. 第2日，抗原品質分析和報告
Day ~3, collecting 5 ml of peripheral blood (pre-immunization) and storage of serum. 第3日，收集5ml外周血(免疫前)，保存血清。
Day ~3, 1st immunization. 第3日，第一次免疫
Day ~17, 2nd immunization. 第17日，第二次免疫
Day ~30, collecting 5ml of peripheral blood and storage of serum. 第30日，收集5ml外周血，保存血清。
Day ~30, 3rd immunization. 第30日，第三次免疫
Day ~45, collecting 5 ml of peripheral blood and storage of serum. 第45日，收集5ml外周血，保存血清。
Day ~45, 4th immunization. 第45日，第四次免疫
Day ~50, collecting 50 ml of peripheral blood, storage of serum and PBMC. 第50日，收集5ml外周血，保存血清。
Day ~55, analysis of serum titer and analysis report. 第55日，血清效價分析和輸出報告
Antigen
抗原
The antigen, including proteins, peptides, DNA plasmids, cells, can be provided either by the customer or by Jotbody upon customer request. 抗原包括蛋白質、多肽、DNA質粒、細胞等，可由客戶提供，也可根據客戶要求由Jotbody提供。
Note: every antigen requires specific guideline. For antigen preparation, ask us for more details. 注：每種抗原都需要專門的指導。 如需製備抗原，請向我們詢問更多細節。
General requirements for antigen proteins Purity: > 90% purity for protein and > 95% for peptide with length > 12 amino acids. Concentration: >1 mg/mL without toxic components like azide. Amount: 4 mg for 2 camelids, 10 mg for 5 sharks. Low immunogenicity antigen might require additional amount. Shipping: packaging at 4 °C when shipping. 抗原品質要求：蛋白純度>90%，多肽純度>95%且長度大於12個氨基酸。 濃度> 1mg /mL，無疊氮化鈉等有毒成分。 用量：2只駱駝4毫克，5只鯊魚10毫克。 低免疫原性抗原可能需要額外的量。 運輸：低溫（4 °C或≤-20°C）包裝和運輸。
Every step is customizable
每一步都可定制
Jotbody 