Jotbody has succeeded in generating single domain antibody from alpaca, camel, llama and shark
Why use Immunized sdAb Phage Library
為什麼我們使用納米抗體噬菌體免疫庫
Specific sdAb can be isolated from an immune library or a naïve library. Without any doubt, the sampling of sdAb derived from an immune library offers better performances. Indeed, the somatic maturation in lymphocytes of immunized alpaca, camel, llama or shark performs a natural selection of variant sdAb upon immunization, that finally will generate sdAb libraries with excellent specificity and affinity toward the antigens of interest. However, we need to consider that, the animal immunization makes the development of Immunized sdAb Phage Library lengthy.
特異性的納米抗體可以從免疫庫或天然庫中分離出來。毫無疑問，從免疫庫中提取的納米抗體具有更好的性能。事實上，免疫後的羊駝、駱駝、美洲駝或鯊魚的淋巴細胞體細胞成熟對變異納米抗體進行了自然選擇，最終生成對抗原具有極好特異性和親和力的納米抗體庫。然而，我們需要考慮的是，動物免疫使得免疫納米抗體噬菌體庫的開發時間變長。
Standard workflow for Immunized sdAb Phage Library construction
納米抗體噬菌體免疫庫構建的標準工作流程
Jotbody has successully constructed single domain antibody libraries via immunization of camel, alpaca, llama or shark.
Jotbody通過免疫駱駝、羊駝、美洲駝或鯊魚，成功構建了納米抗體庫。
| Time 時間 | Note 事項 |
|---|---|
| Day 1~2 第1~2日 | Isolation of total RNA of PBMC PBMC和總RNA分離 |
| Day 3 第3日 | cDNA synthesis cDNA合成 |
| Day 4 第4日 | DNA amplification DNA擴增 |
| Day 5~11 第5~11日 | Construction of sdAb library in phage display vector 噬菌體展示納米抗體文庫的構建 |
Jotbody 