Antibody validation

At Jotbody, our team is dedicated to providing reliable and comprehensive antibody validation to ensure the accuracy and reproducibility of your research results. We pride ourselves on our rigorous antibody validation process, understanding the importance of antibody validation in obtaining accurate and reliable data.

Our comprehensive validation process includes testing for specificity, sensitivity, reproducibility, cross-reactivity, and non-specific binding. We employ various methods, such as Western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), ELISA, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and peptide arrays, to validate antibodies for specific applications.

Specificity is crucial, ensuring that an antibody binds only to its intended target without cross-reacting with other proteins or molecules. Sensitivity determines the ability of an antibody to detect low concentrations of its target. Reproducibility ensures consistent results across different experiments and over time. Additionally, we verify that antibodies are free from non-specific binding and cross-reactivity, preventing unwanted interactions with unrelated targets.

We offer a range of antibody validation services tailored to your specific needs. Our primary antibody validation service focuses on specificity and sensitivity testing. The secondary antibody validation service assesses cross-reactivity and non-specific binding. For unique requirements, our custom antibody validation service can be customized to meet your research needs effectively.

Our team consists of experienced scientists committed to delivering reliable and effective antibody validation services. Whether you require primary antibody validation, secondary antibody validation, or custom antibody validation, we are here to support your research. Contact us today to learn more about our antibody validation services or to place an order.

AssayPurpose/FunctionKey points
ChIPAnalysis of protein-DNA interactions and identification of chromatin modificationsStudy of DNA-binding proteins and epigenetic regulation
ELISALarge-scale protein quantification and screeningQuantitative, high throughput
IFRapid detection and visualization of specific proteins in cells and tissuesHigh throughput, easy to optimize
IHC/ICCVisualization and localization of specific proteins in tissues and cellsReadily available
IPImmunoprecipitation of target proteins for downstream analysis Specific enrichment of protein complexes or interactions
Knockout modelsStudy of gene function by comparing wild-type and knockout modelsProvides best negative controls, applicable to all assays
MSTMeasurement of biomolecular interactions including protein-protein or protein-small molecule binding. Rapid assays with low sample requirements and versatile labeling optionsRapid assay, low sample consumption and volume, immobilization-free, optional labeling
MSProtein identification, characterization, and quantificationConfirms specificity, high throughput
Peptide arraysHigh-throughput screening of peptide-protein interactionsProbe specific peptide-protein interactions
siRNA knockdownTargeted gene knockdown to investigate specific gene functionCan be used in all assays, transient
SPRReal-time measurement of biomolecular interactions without the need for labelsReal-time analysis, label-free, highly sensitive
Western blotProtein detection and analysis in complex mixturesEasy to use, ideal for denatured proteins