Antibody validation
At Jotbody, our team is dedicated to providing reliable and comprehensive antibody validation to ensure the accuracy and reproducibility of your research results. We pride ourselves on our rigorous antibody validation process, understanding the importance of antibody validation in obtaining accurate and reliable data.
Our comprehensive validation process includes testing for specificity, sensitivity, reproducibility, cross-reactivity, and non-specific binding. We employ various methods, such as Western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), ELISA, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and peptide arrays, to validate antibodies for specific applications.
Specificity is crucial, ensuring that an antibody binds only to its intended target without cross-reacting with other proteins or molecules. Sensitivity determines the ability of an antibody to detect low concentrations of its target. Reproducibility ensures consistent results across different experiments and over time. Additionally, we verify that antibodies are free from non-specific binding and cross-reactivity, preventing unwanted interactions with unrelated targets.
We offer a range of antibody validation services tailored to your specific needs. Our primary antibody validation service focuses on specificity and sensitivity testing. The secondary antibody validation service assesses cross-reactivity and non-specific binding. For unique requirements, our custom antibody validation service can be customized to meet your research needs effectively.
Our team consists of experienced scientists committed to delivering reliable and effective antibody validation services. Whether you require primary antibody validation, secondary antibody validation, or custom antibody validation, we are here to support your research. Contact us today to learn more about our antibody validation services or to place an order.
| Assay | Purpose/Function | Key points |
| ChIP | Analysis of protein-DNA interactions and identification of chromatin modifications | Study of DNA-binding proteins and epigenetic regulation |
| ELISA | Large-scale protein quantification and screening | Quantitative, high throughput |
| IF | Rapid detection and visualization of specific proteins in cells and tissues | High throughput, easy to optimize |
| IHC/ICC | Visualization and localization of specific proteins in tissues and cells | Readily available |
| IP | Immunoprecipitation of target proteins for downstream analysis | Specific enrichment of protein complexes or interactions |
| Knockout models | Study of gene function by comparing wild-type and knockout models | Provides best negative controls, applicable to all assays |
| MST | Measurement of biomolecular interactions including protein-protein or protein-small molecule binding. Rapid assays with low sample requirements and versatile labeling options | Rapid assay, low sample consumption and volume, immobilization-free, optional labeling |
| MS | Protein identification, characterization, and quantification | Confirms specificity, high throughput |
| Peptide arrays | High-throughput screening of peptide-protein interactions | Probe specific peptide-protein interactions |
| siRNA knockdown | Targeted gene knockdown to investigate specific gene function | Can be used in all assays, transient |
| SPR | Real-time measurement of biomolecular interactions without the need for labels | Real-time analysis, label-free, highly sensitive |
| Western blot | Protein detection and analysis in complex mixtures | Easy to use, ideal for denatured proteins |