ELISA kits development

Jotbody offers comprehensive ELISA assay development services that cover the entire development process, including target selection, antibody selection and pairing, ELISA optimization, validation, and manufacturing.

Our custom assay development service is tailored to deliver personalized solutions for detecting and quantifying specific biomolecules, and involves the following stages:

Target selection
The first step in ELISA kit development is to identify the target biomolecule of interest. This can be a protein, peptide, or small molecule.

Antibody production, conjugation, and pairing
After identifying the target biomolecule, we can produce the appropriate nanobodies for the ELISA kit. We also offer the option of conjugating a detecting nanobody or using a pre-conjugated secondary nanobody. We then determine and develop the best-matched pairs of antibodies based on sensitivity and specificity, working in tandem with the antibody development processes.

Assay optimization
Once the nanobodies are selected and paired, we proceed to develop a fully optimized ELISA protocol. This includes the selection of appropriate reagents and the optimization of assay conditions, such as incubation times and temperatures.

The ELISA kit is then validated to ensure that it meets the desired specifications, including sensitivity, specificity, and dynamic range. This involves testing the ELISA kit using a range of concentrations of the target biomolecule, to ensure accurate and reliable results.

The ELISA kit is then manufactured in large quantities, ensuring that it is available for use in research applications.

Quality control
The ELISA kits are subjected to rigorous quality control measures to ensure that they meet the desired specifications for accuracy and reliability.

Our custom ELISA assay development service is fully customizable to meet the specific needs of our clients. We work closely with our clients to understand their research goals and provide customized solutions that meet their requirements. Contact us today to learn more about our ELISA assay development service and how we can support your research needs.